Nucleotide sequence of leucine transfer RNA 1 from Candida (Torulopsis) utilis

Two species of 32P-labelled leucine tRNA were highly purified from Candida (Torulopsis) utilis by successive column chromatographies. The purified major species of leucine tRNA 1 was completely digested with ribonuclease T1 [EC 3.1.4.8] and with pancreatic ribonuclease A [EC 3.1.4.22]. The resulting fragments were fractionated, and their nucleotide sequences were determined according to Barrell (1). The results of analyses of the two ribonuclease digests were consistent with each other, and indicated that this tRNA is composed of 85 nucleotide residues, including 14 modified nucleotides. A tentative total sequence has been derived on the basis of several features in the cloverleaf structure for tRNA.