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Single-cell sequencing and its applications in bladder cancer
Wang Wei1,2,3, Yuan Rong1, Liu Sanhe4
1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan430071, People's Republic of China.
Expert Reviews in Molecular Medicine
|January 28, 2022
Summary
Single-cell sequencing (SCS) reveals bladder cancer's complex cellular diversity. This technology enhances understanding of tumour biology, aiding in developing novel biomarkers and targeted therapies for improved patient outcomes.
Area of Science:
- Oncology
- Genomics
- Biotechnology
Background:
- Bladder cancer exhibits significant intra-tumoural heterogeneity.
- Previous large-scale sequencing masked cellular diversity by averaging signals.
- Single-cell sequencing (SCS) offers a higher resolution to study tumour biology.
Purpose of the Study:
- To review advances in single-cell technologies.
- To discuss the application of SCS in bladder cancer research.
- To highlight the potential of SCS in diagnosis and treatment.
Main Methods:
- Review of recent advances in single-cell sequencing technologies.
- Analysis of SCS applications in identifying cellular subpopulations and tumour microenvironments.
- Examination of SCS in characterizing cellular genomic mutations.
Main Results:
- SCS can resolve complex heterogeneity in bladder tumours.
- Distinct cellular subpopulations and their genomic mutations can be identified.
- SCS provides insights into the tumour microenvironment.
Conclusions:
- SCS technologies are crucial for understanding bladder cancer's nuanced biology.
- SCS can identify novel biomarkers and targeted therapeutics.
- SCS has the potential to significantly improve bladder cancer diagnosis and treatment.
