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Whey fermented by Enterococcus faecalis M157 exhibits antiinflammatory and antibiofilm activities against oral

Dahyun Song1, Han Bin Lee1, Geun-Bae Kim2

  • 1Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea.

Journal of Dairy Science
|January 28, 2022
PubMed

Insights

Fermented whey (M157-W) exhibits potent anti-inflammatory and anti-biofilm properties against oral pathogens like Porphyromonas gingivalis and Streptococcus mutans. This study demonstrates its potential for developing novel oral health therapeutics.

Area of Science:

  • Oral Microbiology
  • Immunology
  • Biochemistry

Background:

  • Oral pathogenic bacteria contribute to inflammatory diseases like periodontitis.
  • Bacterial biofilms, particularly Streptococcus mutans, are central to dental caries.
  • Enterococcus faecalis fermentation of whey may yield bioactive compounds.

Purpose of the Study:

  • To evaluate the anti-inflammatory and anti-biofilm effects of Enterococcus faecalis M157-fermented whey (M157-W).
  • To assess M157-W's impact on inflammatory mediators and signaling pathways.
  • To investigate M157-W's efficacy against Streptococcus mutans biofilm formation.

Main Methods:

  • Assessed M157-W's inhibition of lipopolysaccharide (LPS)-induced inflammatory cytokines (IL-1β, IL-6, nitric oxide) in RAW 264.7 cells.
  • Evaluated M157-W's effect on IL-1β and IL-8 production in human periodontal ligament cells.
  • Analyzed M157-W's impact on MAPK and NF-κB signaling pathways.
  • Quantified M157-W's dose-dependent inhibition of Streptococcus mutans biofilm formation and expression of biofilm-related genes (gtfB, gtfC, gtfD).

Main Results:

  • M157-W significantly inhibited LPS-induced IL-1β, IL-6, and nitric oxide in RAW 264.7 cells.
  • M157-W reduced IL-1β and IL-8 production in human periodontal ligament cells.
  • M157-W suppressed MAPK phosphorylation and NF-κB activation.
  • M157-W dose-dependently inhibited Streptococcus mutans biofilm formation and suppressed gtfB, gtfC, and gtfD gene expression.

Conclusions:

  • M157-W possesses significant anti-inflammatory activity against oral pathogens.
  • M157-W effectively inhibits Streptococcus mutans biofilm formation by reducing extracellular polymeric substance synthesis.
  • M157-W demonstrates potential as a therapeutic agent for oral inflammatory conditions and biofilm-related diseases.
Keywords:
Enterococcus faecalisfermentationoral diseasewhey

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