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Area of Science:

  • Neuroscience
  • Optical Imaging
  • Microscopy

Background:

  • Conventional two-photon microscopy has limitations in field-of-view size and excitation volume shape.
  • These constraints restrict the scope of biological questions and obtainable information in neuronal activity monitoring.

Purpose of the Study:

  • To present a low-cost, flexible solution to overcome the limitations of conventional two-photon microscopy.
  • To expand the three-dimensional field-of-view and enable advanced imaging capabilities.

Main Methods:

  • Utilized a non-telecentric optical design.
  • Incorporated rapid laser-focus control using an electrically tunable lens.
  • Developed a system easily implemented and reversible within hours.

Main Results:

  • Achieved a several-fold expansion of the three-dimensional field-of-view.
  • Enabled near-simultaneous imaging of remote 3D regions.
  • Demonstrated the ability to bend imaging planes to follow biological curvatures.
  • Successfully imaged neuronal activity in zebrafish, mice, and fruit flies.

Conclusions:

  • The presented system offers a flexible and cost-effective advancement for two-photon microscopy.
  • The expanded imaging capabilities facilitate a broader range of neuroscience research.
  • The adaptable design serves as a valuable platform for future developments in optical monitoring.