Related Experiment Video
Updated: Oct 5, 2025
10:32
On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
24.7K
A Brief Talk on the Design of Biopharmaceutical Separation and Purification Technology Course
Ying He1, Marvin White2, Wu Gang3
1Institute of Chemical and Pharmaceutical Engineering, Changzhou Vacational Institute of Engineering, Changzhou, Jiangsu 213164, China.
Journal of Healthcare Engineering
|January 28, 2022
Summary
Optimizing fermentation medium significantly enhances the production of bioactive compounds. This bioprocess improvement resulted in a 35% increase in alpha-glucosidase inhibition for biopharmaceutical separation and purification.
Area of Science:
- Biotechnology
- Biopharmaceutical Industry
- Separation and Purification Technologies
Background:
- Biological separation and purification are fundamental to modern biotechnology and the pharmaceutical industry.
- South Korea's biopharmaceutical sector shows significant growth, with advancements in equipment and technology.
- Effective training in biopharmaceutical separation and purification is crucial.
Purpose of the Study:
- To discuss the design of biopharmaceutical separation and purification technology courses.
- To optimize a fermentation process for enhanced production of specific metabolites.
- To evaluate the impact of process optimization on the bioactivity of the purified compounds.
Main Methods:
- Analysis of secondary metabolite synthesis potential in a microbial strain.
- Optimization of the fermentation medium using alpha-glucosidase inhibition rate as an indicator.
- Batch fermentation followed by separation and purification of metabolites.
Main Results:
- The fermentation medium was successfully optimized based on the alpha-glucosidase inhibition rate.
- Batch fermentation was performed using the optimized medium.
- Purified metabolites from the optimized medium showed a significant increase in bioactivity.
Conclusions:
- Optimized fermentation medium significantly boosts the production of valuable secondary metabolites.
- The study achieved a 35% higher alpha-glucosidase inhibition rate with the crude extract from the optimized medium compared to the initial medium.
- This highlights the importance of medium optimization in biopharmaceutical production and purification.
Related Concept Videos
01:20Overview Of Cell Separation And Isolation
6.4K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.4K
01:03Affinity Chromatography
1.8K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
1.8K
01:15Optimizing Chromatographic Separations
533
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
533
01:15Dialysis
872
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
872
01:29Types Of Column Chromatography
12.0K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Gel Filtration Chromatography
When the...
12.0K
01:05Centrifugation
2.7K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.7K