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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Oct 5, 2025

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

Approach for patch-clamping using an upright microscope with z-axis movable stage.

Mina Perić¹, Danijela Bataveljić¹, Dunja Bijelić¹

¹University of Belgrade, Faculty of Biology, Institute of Physiology and Biochemistry "Ivan Djaja", Center for Laser Microscopy, Belgrade, Serbia.

Microscopy Research and Technique

|January 28, 2022

Summary

Researchers developed a novel upright microscope and patch-clamp setup for studying live cell physiology. This cost-effective configuration enables stable recordings and high success rates in electrophysiology experiments.

Keywords:

cellular physiology glial cells life science microscopy patch-clamp

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Area of Science:

Cellular Physiology
Electrophysiology
Microscopy Techniques

Background:

Standard electrophysiology setups require microscopes with fixed stages and movable objectives.
This limits flexibility and can increase equipment costs.

Purpose of the Study:

To present a novel upright microscope and patch-clamp configuration.
To demonstrate its efficacy for studying live cell physiology, particularly glial cells.
To provide a cost-effective and adaptable alternative to commercial systems.

Main Methods:

Utilized an upright microscope with a z-axis movable stage and a fixed objective for patch-clamp experiments.
Developed underlying principles for this configuration.
Tested the system on cultured astrocytes, microglia, and oligodendrocytes.

Main Results:

Achieved stable electrophysiological recordings.
Demonstrated a high success rate for whole-cell patch-clamp trials.
Showcased comparable performance and usability to commercial systems.

Conclusions:

The custom upright microscope/patch-clamp configuration is effective for studying glial cell physiology.
The system is cost-effective, easily replicable, and overcomes equipment limitations.
This approach facilitates routine live cell physiology studies.