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Updated: Oct 5, 2025

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts
Romana Köszagová1,2, Eva Hrabárová1,2, Lucia Achbergerová1,2
1Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Abstract:
Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (D-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase, UDP-glucose pyrophosphorylase) physiologically aggregated within inclusion bodies retaining their biological activity as immobilized biocatalysts.
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