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Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Recursive splicing is a rare event in the mouse brain.

Sohyun Moon¹, Ying-Tao Zhao¹

¹Department of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, New York, United States of America.

|January 28, 2022

Summary

Recursive splicing (RS) is rare in the mouse brain, with only 20 sites found. This study reveals RS is constitutive between neuron types and sexes, and influenced by sequence context.

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Area of Science:

Molecular Biology
Genetics
Neuroscience

Background:

Recursive splicing (RS) removes long introns from mRNA precursors.
RS is well-documented in humans and drosophila but poorly characterized in mice.
The mouse brain is rich in long genes, making it a key area for RS investigation.

Purpose of the Study:

To investigate the prevalence and characteristics of recursive splicing (RS) in the mouse brain.
To determine if RS differs between neuronal subtypes or sexes.
To explore the sequence context influencing RS site selection.

Main Methods:

Nuclear total RNA sequencing was employed to analyze gene expression in the mouse cerebral cortex.
1.15 billion uniquely mapped reads were analyzed to identify RS events.
Sequence analysis was performed to identify motifs associated with RS sites.

Main Results:

Only 20 RS sites were identified in the mouse cerebral cortex, indicating RS is rare in this tissue.
RS was found to be constitutive across excitatory and inhibitory neurons and between sexes.
The primary sequence context near AGGT sites was found to be crucial for distinguishing RS from non-RS sites.
Evidence suggests cryptic exons may utilize an RS-like splicing mechanism.

Conclusions:

Recursive splicing is a rare event in the mouse brain, despite the prevalence of long genes.
RS in the mouse brain is largely constitutive and independent of cell type or sex.
Primary sequence context plays a significant role in the regulation of RS.
RS-like mechanisms may be involved in cryptic exon splicing.