Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A one-pot biplex RPA-Cas assay for sensitive detection of Mycobacterium tuberculosis from tongue swabs.

Scientific reports·2026
Same author

Correction: Room temperature CRISPR diagnostics for low-resource settings.

Scientific reports·2025
Same author

Room temperature CRISPR diagnostics for low-resource settings.

Scientific reports·2025
Same author

Urine lipoarabinomannan concentrations among HIV-negative adults with pulmonary or extrapulmonary tuberculosis disease in Vietnam.

PLOS global public health·2024
Same author

New Manual Quantitative Polymerase Chain Reaction Assay Validated on Tongue Swabs Collected and Processed in Uganda Shows Sensitivity That Rivals Sputum-based Molecular Tuberculosis Diagnostics.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2024
Same author

Correction: Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis.

PloS one·2024

Related Experiment Video

Updated: Oct 5, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.6K

Automated liquid handling robot for rapid lateral flow assay development.

Caitlin E Anderson1, Toan Huynh2, David J Gasperino2

  • 1Global Health Labs, Bellevue, WA, USA. caitlin.anderson@ghlabs.org.

Analytical and Bioanalytical Chemistry
|January 29, 2022
PubMed
Summary

A new automated robotic platform significantly speeds up the development of sensitive and specific lateral flow assays (LFAs). This innovation reduces costs and hands-on time for point-of-care diagnostic test creation.

Keywords:
High-throughput screeningLateral flow assayOptimization

More Related Videos

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

10.9K
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
11:58

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

Published on: April 30, 2018

6.8K

Related Experiment Videos

Last Updated: Oct 5, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.6K
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

10.9K
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
11:58

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

Published on: April 30, 2018

6.8K

Area of Science:

  • Biotechnology
  • Assay Development
  • Point-of-Care Diagnostics

Background:

  • Lateral flow assays (LFAs) are widely used in point-of-care diagnostics due to their cost-effectiveness and ease of use.
  • Developing sensitive and specific LFAs is time-consuming and resource-intensive, often involving labor-intensive workflows.
  • Existing development processes can be a bottleneck, delaying the availability of crucial diagnostic tests.

Purpose of the Study:

  • To introduce a novel automated robotic platform to accelerate and improve the lateral flow assay (LFA) development process.
  • To reduce the cost, time, and labor associated with creating sensitive and specific LFAs.
  • To enable high-throughput optimization of LFA parameters.

Main Methods:

  • Development of an LFA-specific automated robotic liquid handling system with tailored hardware and software.
  • Implementation of large-scale optimization experiments for variables like antibody pairs and reagent concentrations directly on LFAs.
  • Validation of the platform using malaria, SARS-CoV-2, and Mycobacterium tuberculosis LFAs, comparing results to traditional ELISA-like methods.

Main Results:

  • The robotic platform demonstrated significant improvements in assay development efficiency and quality.
  • Minimized hands-on time and maximized experiment size, leading to enhanced reproducibility.
  • Successful application in developing LFAs for diverse targets, including infectious diseases.

Conclusions:

  • The automated robotic platform offers a flexible, rapid, and high-throughput solution for LFA development.
  • This approach substantially improves the quality and quantity of assay development efforts.
  • The system is validated and applicable to a range of diagnostic targets, streamlining point-of-care test creation.