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microRNA-144 modulates the NF-κB pathway in miiuy croaker (Miichthys miiuy) by targeting IκBα gene

Liyuan Yang1, Weiwei Zheng1, Xing Lv1

  • 1Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.

Developmental and Comparative Immunology
|January 29, 2022
PubMed

Insights

MicroRNAs (miRNAs) regulate immune responses. This study shows miR-144 inhibits IκBα, activating the NF-κB pathway and influencing inflammatory responses in fish.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of cellular processes, including immune responses.
  • The NF-κB signaling pathway plays a critical role in inflammation and immunity.
  • Understanding miRNA-mediated regulation is crucial for controlling immune responses, particularly in fish.

Purpose of the Study:

  • To investigate the role of miR-144 in regulating the IκBα/NF-κB signaling pathway.
  • To elucidate the mechanism by which miR-144 affects NF-κB activation.
  • To explore the implications for understanding fish immune responses to pathogens.

Main Methods:

  • Investigated the interaction between miR-144 and IκBα.
  • Analyzed the effect of miR-144 on IκBα expression at the post-transcriptional level.
  • Assessed the impact of miR-144-mediated IκBα inhibition on NF-κB pathway activation.

Main Results:

  • miR-144 binds to the 3' UTR of IκBα, repressing its expression post-transcriptionally.
  • Inhibition of IκBα by miR-144 promotes the activation of the NF-κB signaling pathway.
  • miR-144 influences the nuclear translocation of p65 by modulating IκBα levels.

Conclusions:

  • miR-144 acts as a negative regulator of IκBα, thereby promoting NF-κB pathway activation.
  • This regulatory mechanism highlights the role of miRNAs in controlling inflammatory responses in fish.
  • Further understanding of miR-144 function can aid in managing autoimmune diseases and pathogen responses in fish.
Keywords:
Innate immuneIκBαNF-κB signalingNegatively regulationmicroRNA

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