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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
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Absolute quantification of proteins using element mass spectrometry and generic standards
Alicia Jiménez Nosti1, Laura Cid Barrio1, Francisco Calderón Celis1
1Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain.
Journal of Proteomics
|January 29, 2022
Summary
Elemental mass spectrometry, specifically inductively coupled plasma mass spectrometry (ICP-MS), offers accurate absolute protein quantification without protein standards. Recent advances enable its integration into proteomics workflows, overcoming previous limitations.
Area of Science:
- Analytical Chemistry
- Proteomics
- Biochemistry
Background:
- Elemental mass spectrometry, particularly ICP-MS, is established in inorganic analysis but underutilized in life sciences like proteomics.
- Traditional ICP-MS faced instrumental and analytical limitations for protein analysis.
Purpose of the Study:
- To discuss how ICP-MS limitations have been overcome for protein quantification.
- To highlight necessary improvements and explore existing/potential applications of ICP-MS in absolute quantitative proteomics.
Main Methods:
- Review of instrumental and methodological advances in ICP-MS for proteomics.
- Discussion of ICP-MS compatibility with top-down proteomics and LC-MS workflows.
Main Results:
- ICP-MS can provide absolute quantification of proteoforms using generic sulfur or phosphorus compounds, without specific protein standards.
- ICP-MS applications extend beyond standard certification to complex mixture quantification.
Conclusions:
- ICP-MS is a powerful complementary tool for molecular mass spectrometry in absolute quantitative proteomics, addressing standardization and accuracy challenges.
- Synergistic combination of ICP-MS with LC-MS methodologies facilitates its implementation in quantitative proteomics.
Keywords:
Absolute quantificationElemental mass spectrometryHPLC-ICP-MSICP-MSPost-translational modifications quantification.Protein quantificationMore Related Videos
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