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A Drosophila insulator interacting protein suppresses enhancer-blocking function and modulates replication timing.

Emily C Stow1, James R Simmons1, Ran An1

  • 1Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996, USA.

Gene
|January 29, 2022
PubMed
Summary

HP1 and insulator partner protein 1 (HIPP1) interacts with the Suppressor of Hairy wing [Su(Hw)] insulator complex. HIPP1 influences cell cycle progression and enhancer blocking, but its crotonase-like domain is not involved in enhancer blocking.

Keywords:
CohesinCrotonase -like domainGenome organizationGypsyHIPP1Su(Hw)

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Area of Science:

  • * Molecular Biology
  • * Genomics
  • * Cell Biology

Background:

  • * Insulators are crucial for eukaryotic genome structure and function.
  • * Insulator protein interactions determine insulator site properties.
  • * The roles of insulator partners in Drosophila and their functional specificity are not well understood.

Purpose of the Study:

  • * To investigate the role of HP1 and insulator partner protein 1 (HIPP1) in the Suppressor of Hairy wing [Su(Hw)] insulator complex.
  • * To determine how HIPP1 contributes to Su(Hw) insulator complex function.
  • * To elucidate the involvement of HIPP1's crotonase-like domain (CLD) in insulator activity and cell cycle progression.

Main Methods:

  • * Colocalization studies of HIPP1 and Su(Hw) in polytene chromatin and stress-induced insulator bodies.
  • * Overexpression experiments of HIPP1 and Su(Hw).
  • * Mutation analysis of the HIPP1 CLD.
  • * Assessment of DNA replication progression and enhancer-blocking activity.

Main Results:

  • * HIPP1 colocalizes with the Su(Hw) insulator complex.
  • * Overexpression of HIPP1 or Su(Hw), or mutation of HIPP1 CLD, impairs cell proliferation by limiting DNA replication.
  • * HIPP1 overexpression suppresses Su(Hw) enhancer-blocking function.
  • * Mutation of the HIPP1 CLD does not affect Su(Hw) enhancer blocking.

Conclusions:

  • * HIPP1 is functionally related to the Su(Hw) insulator complex.
  • * HIPP1's CLD influences cell cycle progression but not enhancer blocking.
  • * The study reveals distinct roles for HIPP1 in insulator function and cell cycle regulation.