Related Experiment Video
Updated: Oct 5, 2025
Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
One DNA Methylation Regulates CHIP Gene Expression of Human Breast Cancer and Predicts Recurrence
Tatsuyuki Gohno1, Toru Hanamura2, Masafumi Kurosumi3
1Department of Molecular and Functional Dynamics, Graduate School of Medicine, Tohoku University, Sendai, Japan; gohno717@gmail.com.
Background/Aim:
Carboxyl terminus of Hsc70-interacting protein (CHIP) is a ubiquitin ligase that induces ubiquitination and degradation of its target proteins including oncoproteins. We reported that its down-regulation is associated with tumor progression and metastasis of breast cancer. However, the mechanism through which CHIP gene affects cancer cells is unclear.
Materials And Methods:
We extracted RNA from 45 primary breast cancer samples and compared CHIP mRNA expression profiles, promoter DNA methylation status, and clinicopathological information.
Results:
CHIP mRNA expression was significantly correlated with the tumor progression status. In several samples, a pinpoint CpG methylation in the CHIP gene promoter region was significantly correlated with CHIP mRNA expression. When this specific CpG was methylated in estrogen receptor (ER)-positive cases, a significant difference in 5-year recurrence was not found compared with ER-negative cases.
Conclusion:
CpG methylation contributes to the long-term prognosis of ER-positive breast cancer.
Insights
CpG methylation in the CHIP gene promoter is linked to breast cancer progression. Methylation impacts the prognosis of estrogen receptor-positive breast cancer, affecting long-term outcomes.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- Carboxyl terminus of Hsc70-interacting protein (CHIP) acts as a ubiquitin ligase, degrading oncoproteins.
- CHIP down-regulation correlates with breast cancer progression and metastasis.
- The precise mechanism of CHIP's influence on cancer cells remains unclear.
Purpose of the Study:
- To investigate the role of CHIP gene expression and its promoter methylation in breast cancer.
- To explore the correlation between CHIP methylation, mRNA expression, and clinicopathological features.
- To determine the impact of CHIP methylation on the prognosis of breast cancer patients.
Main Methods:
- Analysis of CHIP mRNA expression profiles in 45 primary breast cancer samples.
- Assessment of CHIP gene promoter DNA methylation status.
- Correlation of molecular data with clinicopathological information and patient outcomes.
Main Results:
- CHIP mRNA expression significantly correlated with tumor progression status.
- Specific CpG methylation in the CHIP promoter region was associated with CHIP mRNA levels.
- In ER-positive cases, methylation of this CpG site did not lead to a significant difference in 5-year recurrence compared to ER-negative cases.
Conclusions:
- CpG methylation of the CHIP gene promoter is a contributing factor to the long-term prognosis of estrogen receptor-positive breast cancer.
- Epigenetic modifications of CHIP may play a role in breast cancer development and progression.
Related Concept Videos
01:46Epigenetic Regulation
02:30Genomic Imprinting and Inheritance
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...