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Updated: Oct 5, 2025
Mitochondrial Respiration Quantification in Yeast Whole Cells
Published on: November 8, 2024
ICT1 deficiency leads to reduced oxygen resistance due to the cell wall damage in S. cerevisiae
Hong Zhu1, Mengfei Wang1, Hua Zhou1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211800, China.
Background:
Disruption of ICT1 has been known to cause a significant deficiency in the phospholipid composition which is necessary for cell stress adaptation. However, the effects of ICT1 deletion on antioxidant research are not clear.
Objective:
Construct a knockout strain to investigate the efficacy of ICT1 on antioxidant activity.
Methods:
The antioxidant-related genes and phospholipid-related genes were determined by RT-PCR, the cell wall shape was observed by TME, and CWI pathway phosphorylation experiments were also analyzed by HPLC.
Results:
The expression of antioxidant related genes and phospholipid-related genes has a slight reduction compared to the wild type. The cell wall was observed impaired with apparent CWI pathway phosphorylation weakening in the mutant.
Conclusion:
These findings indicate the role of ICT1 on antioxidant activity because it not only directly affects phospholipid composition but also further leads to the activation of CWI.
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