Charge-Based Multiarm Avidin Nanoconstruct as a Platform Technology for Applications in Drug Delivery
Tengfei He1, Chenzhen Zhang1, Ambika G Bajpayee2,3
1Department of Bioengineering, Northeastern University, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2022
Summary
We developed cationic multiarm Avidin (mAv) nanoconstructs for drug delivery and imaging. These constructs enable tunable, sustained drug release via the avidin-biotin reaction for targeted therapeutic applications.
Area of Science:
- Biotechnology
- Nanomedicine
- Drug Delivery Systems
Background:
- Cationic nanoconstructs are valuable for drug delivery and imaging, particularly for targeting negatively charged tissues.
- Avidin-biotin interactions offer a versatile platform for bioconjugation due to their high affinity and specificity.
Purpose of the Study:
- To synthesize cationic multiarm Avidin (mAv) nanoconstructs for enhanced drug delivery and imaging applications.
- To establish a flexible method for conjugating various drugs to the nanoconstructs.
- To enable tunable and sustained drug release kinetics.
Main Methods:
- Synthesis of cationic multiarm Avidin (mAv) nanoconstructs.
- Utilizing the avidin-biotin reaction for drug conjugation at room temperature.
- Controlling ester linker hydrolysis rates to modulate drug release.
Main Results:
- Successfully synthesized mAv nanoconstructs with cationic properties.
- Demonstrated facile and flexible conjugation of biotinylated drugs via the avidin-biotin system.
- Achieved tunable and sustained drug release profiles through controlled linker hydrolysis.
Conclusions:
- Cationic multiarm Avidin nanoconstructs offer a promising platform for targeted drug delivery and imaging.
- The avidin-biotin conjugation strategy provides flexibility for diverse therapeutic applications.
- Tunable drug release kinetics can be achieved, paving the way for optimized therapeutic dosing.
