Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.9K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.9K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

72.7K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
72.7K
Antibiotic Selection00:57

Antibiotic Selection

56.1K
Overview
56.1K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

5.1K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nipple fluid for breast cancer diagnosis using the nanopore of Phi29 DNA-packaging motor.

Nanomedicine : nanotechnology, biology, and medicine·2022
Same author

In vivo peptide-based delivery of a gene-modifying enzyme into cells of the central nervous system.

Science advances·2022
Same author

Photoinduced Covalent Irreversible Inactivation of Proline Dehydrogenase by S-Heterocycles.

ACS chemical biology·2021
Same author

Targeted melanoma radiotherapy using ultrasmall <sup>177</sup>Lu-labeled α-melanocyte stimulating hormone-functionalized core-shell silica nanoparticles.

Biomaterials·2020
Same author

Operative Binding of Class I Release Factors and YaeJ Stabilizes the Ribosome in the Nonrotated State.

Biochemistry·2018
Same author

Targeting aggressive prostate cancer-associated CD44v6 using phage display selected peptides.

Oncotarget·2017

Related Experiment Video

Updated: Oct 5, 2025

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.5K

Selection of Cancer Stem Cell-Targeting Agents Using Bacteriophage Display.

Austin R Prater1, Susan L Deutscher2

  • 1Harry S. Truman Veterans Memorial Hospital and Department of Biochemistry, University of Missouri, Columbia, MO, USA. deutschers@missouri.edu.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2022
PubMed
Summary
This summary is machine-generated.

Targeting cancer stem cells (CSCs) is crucial for aggressive cancers. This study explores using phage display technology, combining in vitro and in vivo methods, to discover novel CSC-targeting agents for improved diagnostics and therapeutics.

Keywords:
BacteriophageBiomarkersCancer stem cellsCharacterization/target validationPeptide-based tumor -targetingScreening

More Related Videos

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
07:32

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Published on: September 30, 2019

7.9K
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.3K

Related Experiment Videos

Last Updated: Oct 5, 2025

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.5K
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
07:32

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Published on: September 30, 2019

7.9K
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.3K

Area of Science:

  • Oncology
  • Biotechnology
  • Molecular Biology

Background:

  • Aggressive cancers often relapse and resist treatment, with cancer stem cells (CSCs) implicated as a primary cause.
  • Targeting CSCs offers a promising avenue for developing new diagnostic and therapeutic strategies.
  • Bacteriophage (phage) display technology provides a powerful platform for identifying molecules that bind to specific targets.

Purpose of the Study:

  • To detail a methodology for isolating cancer stem-like cells (CSCs) from aggressive cancer cell lines.
  • To describe the application of both in vitro and in vivo phage display selections against CSCs.
  • To outline the characterization of phage-displayed peptides for potential diagnostic and therapeutic use.

Main Methods:

  • Isolation of a cancer stem-like cell (CSC) population from aggressive cancer cell lines.
  • Performance of in vitro phage display selections against immobilized CSCs.
  • Performance of in vivo phage display selections within a biological system.
  • Combination of in vitro and in vivo selection strategies to leverage advantages of both methods.
  • Characterization of isolated phage/peptides for CSC targeting efficacy.

Main Results:

  • Successful isolation of a CSC-like cell population.
  • Identification of specific phage/peptides that bind to CSCs through combined selection methods.
  • Demonstration of the feasibility of using phage display for CSC targeting agent discovery.
  • Establishment of a comprehensive workflow for CSC-targeting agent development.

Conclusions:

  • A combined in vitro and in vivo phage display approach is effective for discovering CSC-targeting agents.
  • The identified phage/peptides hold potential for developing novel diagnostic and therapeutic tools for aggressive cancers.
  • This methodology provides a robust framework for advancing CSC-targeted cancer therapies.