Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 5, 2025

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
06:19

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Published on: November 17, 2018

16.8K

A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath.

Ville A Palomäki1, Vesa Koivukangas1, Sanna Meriläinen1

  • 1Department of Surgery, Oulu University Hospital and University of Oulu, Oulu, Finland.

Adipocyte
|January 31, 2022
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor-infiltrating Basophils Are Associated With Improved Prognosis in Colorectal Cancer.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

Tumor Invasive Border Index (TIBI) in colorectal cancer: linking infiltrative morphology to molecular insights.

The Journal of pathology·2026
Same author

Low muscle density is associated with morbidity and systemic inflammation in colorectal cancer, independent of tumor factors.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same author

Acute cell‑death and lysosomal stress responses to nicotine and cigarette smoke extract in human mesenchymal stromal cells.

Archives of toxicology·2026
Same author

Eating behaviors following metabolic bariatric surgery in lower, medium, and higher responders.

Eating behaviors·2026
Same author

Opposing patterns in eating behaviors following bariatric surgery versus lifestyle-induced weight loss.

PloS one·2026

Quantifying fat cell size in obesity research is crucial. A new, straightforward QuPath method accurately measures adipose tissue morphology, correlating well with existing tools for whole-slide analysis.

Area of Science:

  • Adipose tissue biology
  • Histomorphometry
  • Obesity research

Background:

  • Adipose tissue morphology changes, like enlarged fat cells, are hallmarks of overweight and obesity.
  • Fat cell size is linked to low-grade inflammation and metabolic syndrome, including type 2 diabetes.
  • Manual quantification of fat cell morphology from histological images is time-consuming.

Purpose of the Study:

  • To present a simple, efficient method for quantifying human adipose tissue morphology.
  • To validate the accuracy of the proposed method against established image analysis techniques.

Main Methods:

  • Utilized the free, open-source software QuPath exclusively.
  • Employed QuPath's built-in tools for automated measurement of fat cell morphology.
Keywords:
Adipocyte ToolsAdipocyte sizeImageJQupathfat cell sizeobesity

More Related Videos

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
10:10

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

Published on: August 19, 2020

7.7K
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
06:46

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

8.7K

Related Experiment Videos

Last Updated: Oct 5, 2025

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
06:19

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Published on: November 17, 2018

16.8K
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
10:10

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

Published on: August 19, 2020

7.7K
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
06:46

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

8.7K
  • Applied the protocol to human adipose tissue samples for analysis.

    • Main Results:

    • The QuPath method demonstrated excellent correlation with measurements from ImageJ software combined with manual correction.
    • Intraclass correlation coefficients indicated a good to excellent agreement between the two quantification methods.
    • The protocol is suitable for analyzing large tissue areas, including whole-slide imaging.

    Conclusions:

    • A straightforward QuPath-based protocol provides accurate and efficient quantification of adipose tissue morphology.
    • This method offers a reliable alternative for researchers studying obesity and related metabolic diseases.
    • The approach facilitates large-scale analysis of adipocyte size and its clinical implications.