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Updated: Oct 5, 2025
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
MiR-30a-5p hampers proliferation of lung squamous cell carcinoma through targeting FBXO45
Fanye Zeng1, Shuqing You2, Xueli Dai3
1Second Department of Medical Oncology, The Fourth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Objective:
Studies have elaborated the inhibition of miR-30a-5p on the proliferation of cancer cells. However, the regulatory mechanism of how miR-30a-5p works in lung squamous cell carcinoma (LUSC) cells is obscure.
Methods:
Data of miRNAs/mRNAs in LUSC tissue (The Cancer Genome Atlas (TCGA)) were accessed. A differential upstream miRNA (miR-30a-5p) was obtained by differential analysis. Downstream target mRNAs were predicted and screened by several databases. The function pathways of target protein in cells were determined by gene set enrichment analysis (GSEA). Abnormal expression levels of FBXO45 and miR-30a-5p were evaluated in three LUSC cell lines. The expression levels of FBXO45 mRNA and miR-30a-5p were analyzed by qRT-PCR. Western blot method was employed to assess protein levels of FBXO45, Cyclin E1, Cdk4 and Cyclin D1. How the two researched genes interact was testified by dual-luciferase method. Cell proliferative ability was compared by CCK-8 and colony formation methods. Moreover, cell cycle was tested by flow cytometry.
Results:
MiR-30a-5p was tested to be noticeably down-regulated in LUSC cell lines. Up-regulated FBXO45 in LUSC was targeted by miR-30a-5p. Overexpressing miR-30a-5p modulated proliferation and cell cycle in LUSC via inhibiting FBXO45.
Conclusion:
MiR-30a-5p hindered FBXO45 expression to repress the proliferation of LUSC. FBXO45/miR-30a-5p may shed light on future molecular treatment of LUSC.
Insights
MicroRNA-30a-5p (miR-30a-5p) inhibits lung squamous cell carcinoma (LUSC) proliferation by targeting FBXO45. This finding offers potential for novel molecular therapies for LUSC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA-30a-5p (miR-30a-5p) is known to inhibit cancer cell proliferation.
- The specific regulatory mechanisms of miR-30a-5p in lung squamous cell carcinoma (LUSC) remain unclear.
Purpose of the Study:
- To elucidate the role and regulatory mechanism of miR-30a-5p in LUSC.
- To investigate the interaction between miR-30a-5p and its target genes in LUSC cells.
Main Methods:
- Differential expression analysis of miRNA and mRNA data from The Cancer Genome Atlas (TCGA) for LUSC.
- Bioinformatic prediction and screening of downstream target mRNAs for miR-30a-5p.
- Gene Set Enrichment Analysis (GSEA) to determine functional pathways.
- Quantitative real-time PCR (qRT-PCR) and Western blot to assess gene and protein expression levels.
- Dual-luciferase assay, CCK-8, colony formation, and flow cytometry to evaluate gene interaction and cellular functions.
Main Results:
- MiR-30a-5p was significantly downregulated in LUSC cell lines.
- FBXO45 was identified as a direct target of miR-30a-5p and was upregulated in LUSC.
- Overexpression of miR-30a-5p inhibited LUSC cell proliferation and modulated the cell cycle by suppressing FBXO45.
Conclusions:
- MiR-30a-5p acts as a tumor suppressor in LUSC by inhibiting FBXO45 expression, thereby repressing cell proliferation.
- The FBXO45/miR-30a-5p axis presents a potential therapeutic target for LUSC treatment.
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