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Updated: Oct 5, 2025
Spinal Cord Neurons Isolation and Culture from Neonatal Mice
Published on: July 11, 2017
Can chlorogenic acid reduce oxidative stress and in an experimental spinal cord injury?
Ercan Bal1, Şahin Hanalıoğlu2, Aydın Sinan Apaydın3
1Department of Neurosurgery, Ankara Yıldırım Beyazıt University, School of Medicine, Ankara, Turkey.
Background:
We aimed to investigate antioxidant and neuroprotective properties of chlorogenic acid in spinal cord injury (SCI).
Methods:
Twenty-one rats were divided into three groups. Laminectomy was performed in group L (n=7), spinal cord trauma was induced in group T (n=7), and spinal cord trauma was induced and chlorogenic acid treatment was started in group C (n=7). Blood samples were collected to analyze baseline values and the 12th h, 1st day, 3rd day, and 5th day catalase, native thiol (NT), total thiol (TT), disulfide (SS), SS/TT, SS/NT, and NT/TT levels. Functional analysis with Basso-Beattie and Bresnahan scores was performed at the same time points. Total antioxidant status (TAS), total oxidative stress, oxidative stress index, and cyclooxygenase-2 (Cox-2) were examined in the spinal cord of rats euthanized on day 7; results were statistically analyzed.
Results:
On day 7, catalase levels in Group C were significantly higher than baseline levels, whereas those in Group T were significantly lower than baseline levels; Group L showed no significant difference (p=0.008). SS values on day 7 were lower in Group T than in Groups C and L. Group C showed the lowest decrease in NT/TT level after trauma. On day 7, SS/TT level was high in Group T but stable in Groups C and L (p=0.04). Histopathological examination revealed significantly lower Cox-2 and TAS levels in Group C than in Group T (p=0.003, p=0.017, respectively).
Conclusion:
In this study, SCI was primarily examined through thiol-SS balance, and it was demonstrated by experimental models that chlorogenic acid has antioxidant and neuroprotective effects in SCI.
