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Related Experiment Video

Updated: Oct 5, 2025

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Analyzing Lymphatic Vessel Patterning in Adult Tissue.

Louise A Johnson¹

¹MRC Human Immunology Unit, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK. Louise.johnson@imm.ox.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|January 31, 2022

Summary

Whole-mount immunostaining offers a 3D view of intact tissues, ideal for visualizing lymphatic and blood networks. This guide details immunofluorescence staining methods for imaging lymphatic vessels in mouse skin.

Keywords:

Antibodies Blood vessels Confocal microscopy Endothelial cells Immunofluorescence microscopy LYVE-1 Lymphatic vessels Skin Whole-mount

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Last Updated: Oct 5, 2025

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

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Published on: March 8, 2013

Area of Science:

Immunology
Microscopy
Vascular Biology

Background:

Whole-mount immunostaining provides a comprehensive 3D overview of intact tissues.
Traditional sectioning methods offer limited fields of view.
High-resolution confocal microscopy is crucial for detailed vascular imaging.

Purpose of the Study:

To provide a practical guide for whole-mount immunofluorescence staining.
To detail the imaging of lymphatic vessels in mouse skin.
To illustrate the spatial positioning and cellular interactions within vascular networks.

Main Methods:

Whole-mount immunostaining of intact mouse skin.
High-resolution confocal microscopy.
Immunofluorescence staining techniques.

Main Results:

Detailed 3D spatial positioning of lymphatic and blood vessels.
Visualization of subcellular antigen arrangements.
Observation of cellular interactions within the interstitium and vessel lumen.

Conclusions:

Whole-mount immunostaining is an effective technique for studying vascular networks in 3D.
This method enhances the understanding of vessel architecture and cell-tissue interactions.
The provided guide facilitates the application of this technique in research settings.