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A Novel Method to Monitor GABA Loading into Synaptic Vesicles by Combining Patch Pipette Perfusion and Intracellular,
Manami Yamashita1, Tetsuya Hori2
1Department of Physiology, Faculty of Medicine, Osaka Medical and Pharmaceutical University, Osaka, Japan. manami.yamashita@ompu.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|January 31, 2022
Abstract:
A given concentration of GABA can be introduced into a presynaptic terminal by patch clamping the soma of a presynaptic neuron, if the neuron has a relatively short axon. By combining patch pipette perfusion or intracellular, caged-GABA photolysis, it is possible to measure various parameters related to synaptic vesicle filling with GABA.
