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Surface Engineered Peroxidase-Mimicking Gold Nanoparticles to Subside Cell Inflammation
Mamta Kumawat1, Harishkumar Madhyastha2, Akhela Umapathi1
1Amity Center for Nanobiotechnology and Nanomedicine (ACNN), Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur 303002, Rajasthan, India.
Langmuir : the ACS Journal of Surfaces and Colloids
|January 31, 2022
Summary
Curcumin-based gold nanoparticles functionalized with isoniazid, tyrosine, or quercetin exhibit peroxidase-mimicking and antioxidant properties. These biocompatible nanoparticles show potential for nanomedicine by reducing inflammation without significant toxicity.
Area of Science:
- Nanotechnology
- Materials Science
- Biomedical Engineering
Background:
- Conventional nanoparticle synthesis often relies on toxic chemical methods, limiting their biomedical applications.
- Developing safe and effective nanoparticles requires innovative, biocompatible synthesis and functionalization strategies.
- Plant-derived compounds offer a promising alternative for creating functional nanomaterials.
Purpose of the Study:
- To synthesize curcumin-based gold nanoparticles (Au NPs).
- To functionalize Au NPs with isoniazid (INH), tyrosine (Tyr), and quercetin (Qrc) to create surface coronas.
- To evaluate the peroxidase-mimicking, antioxidant, and anti-inflammatory properties of these engineered Au NPs.
Main Methods:
- Curcumin-mediated synthesis of gold nanoparticles.
- Surface functionalization of gold nanoparticles with INH, Tyr, and Qrc.
- Assessment of peroxidase-mimetic activity and free radical scavenging capacity.
- In vitro evaluation of hemocompatibility and biocompatibility using red blood cells and macrophages.
- Quantification of anti-inflammatory effects by measuring pro-inflammatory cytokines (IL-6, TNF-α, IL-1β).
Main Results:
- Curcumin-based gold nanoparticles (Cur-Au) were successfully synthesized and functionalized.
- All engineered nanoparticles (Cur-AuINH, Cur-AuTyr, Cur-AuQrc) displayed peroxidase-mimicking activity and potent free radical scavenging capabilities.
- The nanoparticles demonstrated excellent hemocompatibility and biocompatibility with macrophages.
- Surface-engineered Au NPs effectively reduced key pro-inflammatory cytokines, including IL-6, TNF-α, and IL-1β.
- No significant toxicity was observed for the developed nanoparticles.
Conclusions:
- Curcumin-based gold nanoparticles with tailored surface coronas exhibit promising peroxidase-mimicking and antioxidant properties.
- These engineered nanoparticles are biocompatible and possess significant anti-inflammatory potential.
- The developed nanomaterials offer a non-toxic, plant-derived platform for advanced nanomedicine applications.
