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Differential expression analysis of microRNAs and mRNAs in the mouse hippocampus of post-stroke depression (PSD)
Fan Qinlin1, Xie Qi1, Chen Qiong1
1Department of Neurology, Second Affiliated Hospital of Army Medical University, Chongqing, China.
Abstract:
To clarify the differential expressions of microRNAs and mRNAs in a PSD model, this study employed PSD mice for model construction by injecting vasoconstrictor ET-1 (angioendothelin-1) into the medial prefrontal cortex (mPFC) of mice. The animals underwent elevated plus maze test, open field test, tail suspension test, and forced swimming test subsequently. Transcriptome sequencing was performed to analyze the differentially expressed mRNAs and microRNAs. The results showed that open arm entries and time of PSD mice were markedly decreased. Times of the entry to center for mice in the model group were apparently decreased. The climbing time of mice in the model group was greatly decreased. The behavior of PSD mice indicated a marked change, and several indicators of the behavioral tests were significantly lower than those of the control group. Transcriptome sequencing analysis demonstrated that expressions of 1 206 genes and 21 microRNAs were markedly upregulated in model group, whereas expressions of 2 113 genes and 32 microRNAs were markedly downregulated. GO analysis revealed that the differentially expressed genes were mainly involved in regulatory pathways of single-multicellular organism process, developmental process, cell periphery, plasma membrane, and neuron projection. Meanwhile, KEGG analysis results indicated that the differentially expressed genes mostly participated in signaling pathways of neuroactive ligand-receptor interaction, calcium signaling pathway, and cytokine-cytokine receptor interaction. In conclusion, differentially expressed microRNAs and mRNAs were screened, which offers a theoretical foundation for further investigation of molecular mechanisms and novel insight for the early identification, prevention, and treatment of PSD.
Insights
This study identified distinct microRNA and mRNA expression changes in a post-stroke depression (PSD) mouse model. These findings provide a foundation for understanding PSD
Area of Science:
- Neuroscience
- Molecular Biology
- Genomics
Background:
- Post-stroke depression (PSD) is a common complication after stroke.
- Understanding the molecular mechanisms underlying PSD is crucial for effective treatment.
Purpose of the Study:
- To investigate the differential expression of microRNAs (miRNAs) and messenger RNAs (mRNAs) in a mouse model of PSD.
- To identify potential molecular targets for PSD diagnosis and therapy.
Main Methods:
- A PSD mouse model was established by injecting endothelin-1 (ET-1) into the medial prefrontal cortex (mPFC).
- Behavioral tests (elevated plus maze, open field, tail suspension, forced swimming) were conducted.
- Transcriptome sequencing was used to analyze differential gene and miRNA expression.
Main Results:
- PSD mice exhibited significantly decreased activity in behavioral tests.
- Transcriptome analysis revealed altered expression of 1,206 upregulated and 2,113 downregulated genes, along with 21 upregulated and 32 downregulated miRNAs.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated involvement in neural development, cell signaling, and receptor interactions.
Conclusions:
- This study successfully identified differentially expressed miRNAs and mRNAs in a PSD mouse model.
- The findings offer a theoretical basis for further research into PSD's molecular mechanisms.
- These results provide novel insights for the early identification, prevention, and treatment of PSD.
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