Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Highly frequent undesired insertional mutagenesis during Drosophila genome editing.

PLoS genetics·2026
Same author

Two classes of amine/glutamate multi-transmitter neurons innervate Drosophila internal male reproductive organs.

eLife·2026
Same author

Pre- and postsynaptic upregulation of FasII synergistically underlies neuropathological and behavioral phenotypes in a Drosophila model of myotonic dystrophy.

Nature communications·2025
Same author

Two classes of amine/glutamate multi-transmitter neurons innervate Drosophila internal male reproductive organs.

bioRxiv : the preprint server for biology·2025
Same author

Multimerized epitope tags for high-sensitivity protein detection.

G3 (Bethesda, Md.)·2025
Same author

Synapse-specific catecholaminergic modulation of neuronal glutamate release.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Oct 5, 2025

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

1.0K

A conditional glutamatergic synaptic vesicle marker for Drosophila.

Sarah J Certel1, Evelyne Ruchti2, Brian D McCabe2

  • 1Division of Biological Sciences, Center for Structural and Functional Neuroscience, The University of Montana, Missoula, MT 59812, USA.

G3 (Bethesda, Md.)
|January 31, 2022
PubMed
Summary

Researchers developed smFLAG-vGlut, a new tool to identify glutamate-releasing neurons and their vesicles in fruit flies. This marker aids in understanding brain circuitry and how information processing generates behavior.

Keywords:
Drosophilaepitope tagglutamatergicsynaptic vesiclevGlut

More Related Videos

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
05:58

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

Published on: October 15, 2010

9.6K
Appetitive Associative Olfactory Learning in Drosophila Larvae
09:22

Appetitive Associative Olfactory Learning in Drosophila Larvae

Published on: February 18, 2013

19.2K

Related Experiment Videos

Last Updated: Oct 5, 2025

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

1.0K
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
05:58

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

Published on: October 15, 2010

9.6K
Appetitive Associative Olfactory Learning in Drosophila Larvae
09:22

Appetitive Associative Olfactory Learning in Drosophila Larvae

Published on: February 18, 2013

19.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Glutamate is a key excitatory neurotransmitter vital for nervous system development, synaptic plasticity, learning, and memory.
  • Identifying glutamatergic neurons and their release sites is crucial for understanding neural circuit function and behavior generation.

Purpose of the Study:

  • To develop and characterize smFLAG-vGlut, a conditional marker for glutamatergic synaptic vesicles in Drosophila.
  • To validate the functionality, conditional expression, and specificity of smFLAG-vGlut.

Main Methods:

  • Conditional genetic labeling of synaptic vesicles.
  • Functional validation of the smFLAG-vGlut marker.
  • Glutamatergic neurotransmitter phenotyping of Drosophila central complex neurons.

Main Results:

  • smFLAG-vGlut was successfully validated for functionality, conditional expression, and specificity.
  • Nine out of 26 central complex neuron types were identified as glutamatergic using smFLAG-vGlut.
  • The marker facilitates the identification of glutamate release sites in Drosophila neurons.

Conclusions:

  • smFLAG-vGlut is a reliable tool for identifying glutamatergic neurons and synaptic vesicles in Drosophila.
  • This tool enhances the modeling of neural circuitry, particularly in the central complex.
  • The method is adaptable for use with any Drosophila neuron type accessible via binary transcription system drivers.