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The Novel microRNA Rno-miR-686-3p Is Associated with the Ischaemic Penumbra
Wei Wang1, Jin-Pin Li2, Jing-Li Liu2
1Department of Neurology, The First Affiliated Hospital of Guangxi Medical University in Nanning, Guangxi, China, wangweimedical@163.com.
Introduction:
We explored microRNA (miRNA) profiles correlated with the penumbra in three different phases of ischaemic stroke, using a permanent middle cerebral artery occlusion (p-MCAO) rat model.
Materials And Methods:
A 2-mm coronal section was cut from the optic chiasma in the caudal direction, and the penumbra was located in the area between a longitudinal line approximately 2 mm from the midline and a transverse diagonal line at the "2-o'clock" position. Total RNA was extracted from tissue specimens and peripheral blood samples, followed by deep sequencing analysis.
Results:
We identified nine novel miRNA candidates in tissues and evaluated their expression levels using real-time quantitative polymerase chain reaction. In situ hybridization was conducted to assess miRNA localization in the brain. Of these nine candidates, we identified and characterized a novel miRNA, rno-miR-686-3p, which was localized in cell nuclei of the cortex, and associated with the penumbra. rno-miR-686-3p was downregulated at 1 (p = 0.042), 3 (p = 0.032), and 4 h (p = 0.007) post-p-MCAO in the penumbra. A total of 297 potential target genes were predicted. Moreover, functional annotation clustering and pathway enrichment analysis predicted that rno-miR-686-3p participates in transcriptional regulation and the Wnt and cyclic adenosine monophosphate (cAMP) signalling pathways.
Conclusion:
rno-miR-686-3p is a novel miRNA associated with the ischaemic penumbra that is implicated in transcriptional regulation and modulation of the Wnt and cAMP signalling pathways. Furthermore, it may serve as a possible new biomarker with potential value for detecting the existence of the penumbra.
Insights
Researchers identified a novel microRNA, rno-miR-686-3p, in the penumbra region of ischemic stroke. This microRNA is downregulated after stroke and may serve as a biomarker for stroke detection.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Ischemic stroke poses a significant health burden, with the penumbra representing a critical area for therapeutic intervention.
- Understanding the molecular mechanisms underlying stroke, particularly in the penumbra, is crucial for developing effective treatments.
Purpose of the Study:
- To identify microRNA (miRNA) profiles associated with the ischemic penumbra in a rat model of stroke.
- To characterize novel miRNAs and their potential roles in stroke pathophysiology.
Main Methods:
- Utilized a permanent middle cerebral artery occlusion (p-MCAO) rat model to induce ischemic stroke.
- Performed deep sequencing analysis of RNA from brain tissue and peripheral blood samples.
- Employed real-time quantitative polymerase chain reaction and in situ hybridization to validate and localize miRNA expression.
Main Results:
- Identified nine novel miRNA candidates, including rno-miR-686-3p, localized in the cortical cell nuclei and associated with the penumbra.
- Observed significant downregulation of rno-miR-686-3p in the penumbra at 1, 3, and 4 hours post-p-MCAO.
- Predicted 297 target genes and identified involvement in transcriptional regulation and Wnt/cAMP signaling pathways.
Conclusions:
- rno-miR-686-3p is a novel miRNA linked to the ischemic penumbra, involved in transcriptional regulation and Wnt/cAMP signaling.
- rno-miR-686-3p shows potential as a novel biomarker for detecting the presence of the ischemic penumbra.
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