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Updated: Oct 5, 2025
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Published on: May 22, 2018
Synthesis of carbon quantum dots for application of alleviating amyloid-β mediated neurotoxicity
Haolin Li1, Yufei Zhang1, Jianwei Ding2
1CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Alzheimer's disease (AD) has been an intractable neurodegenerative disease among the elderly in the worldwide. One of the important pathogenic factors in AD is the aggregation of amyloid-β 1-42 (Aβ42) in AD patients' brain. Inhibition of Aβ peptide aggregation is considered as one of the effective approaches against AD. Herein, a pulsed laser ablation (PLA) method is used to fabricate ultra-small carbon quantum dots (C-QDs) with uniform size. The reduced Aβ42 aggregation by the C-QDs was confirmed by transmission electron microscopy (TEM), thioflavin T assay and circular dichroism (CD) spectroscopy. The results of cell viability experiments showed that the presence of the C-QDs could significantly reduce the cytotoxicity of Aβ42. Furthermore, in vivo studies demonstrate that C-QDs can decrease Aβ42 deposits and promote the biological activity of an AD model of Caenorhabditis elegans CL2006. This work demonstrates the viability of using ultrasmall C-QDs to inhibit amyloid-β aggregation and alleviate amyloid-β mediated neurotoxicity.
