Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix
Nicola Facchinello1, Matteo Astone1, Matteo Audano2
1Laboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy.
Nature Metabolism
|February 1, 2022
Summary
Endothelial cell metabolism regulates blood vessel maturation. The oxidative pentose phosphate pathway (oxPPP) is crucial for vascular mural cell (vMC) recruitment and vessel stabilization by controlling elastin production.
Area of Science:
- Vascular Biology
- Cell Metabolism
- Developmental Biology
Background:
- Vascular mural cells (vMCs) are vital for blood vessel development and stabilization.
- The influence of endothelial cell metabolism on vMC recruitment remains unclear.
Purpose of the Study:
- To investigate the role of endothelial cell metabolism in vMC recruitment and differentiation.
- To elucidate the mechanisms by which endothelial metabolism impacts vascular maturation.
Main Methods:
- Utilized zebrafish and mouse models for early vertebrate development studies.
- Investigated the oxidative pentose phosphate pathway (oxPPP) in endothelial cells.
- Assessed the impact of laminar shear stress and blood flow on oxPPP activity.
Main Results:
- Endothelial oxPPP activity is essential for vMC coverage of the dorsal aorta.
- Laminar shear stress and blood flow maintain endothelial oxPPP activity.
- OxPPP promotes elastin expression via ribose-5-phosphate, driving vMC recruitment and maintenance.
Conclusions:
- Endothelial cell metabolism, specifically the oxPPP, is a key regulator of vascular mural cell recruitment.
- The oxPPP influences vascular matrix composition by controlling elastin production.
- This metabolic pathway is critical for blood vessel maturation and stabilization.
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