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Related Experiment Video

Updated: Oct 5, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing.

Macarena Moronta Gines¹, Kerstin S Wendt²

¹Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|February 1, 2022

Summary

This study details a ChIP protocol for mapping cohesin and CTCF binding sites. This method aids in understanding chromatin loop formation and cohesin regulation across mammalian genomes.

Keywords:

ChIP Chromatin immunoprecipitation Cohesin

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Last Updated: Oct 5, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Capturing Chromosome Conformation Across Length Scales

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Published on: January 20, 2023

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Cohesin and CTCF are crucial for genome architecture.
Understanding their binding sites informs chromatin loop formation.
Cohesin regulation is complex and requires detailed study.

Purpose of the Study:

To present a detailed ChIP protocol for cohesin subunits and regulators.
To facilitate the study of cohesin-CTCF collaboration in chromatin looping.
To enable insights into cohesin's intricate regulatory mechanisms.

Main Methods:

Chromatin immunoprecipitation (ChIP) followed by sequencing (ChIP-seq).
Application across various mammalian cell lines.
Adaptable protocol for different cohesin subunits and regulators.

Main Results:

Successfully established a detailed ChIP protocol.
Demonstrated protocol's utility in diverse cell lines.
Protocol enables mapping of cohesin and CTCF binding sites.

Conclusions:

The described ChIP protocol is effective for studying cohesin and CTCF.
This method is fundamental for investigating chromatin loop formation.
The protocol provides a valuable tool for understanding cohesin regulation.