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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

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Infrared Laser Ablation Microsampling with a Reflective Objective.

Chao Dong¹, Luke T Richardson², Touradj Solouki²

¹Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, United States.

Journal of the American Society for Mass Spectrometry

|February 1, 2022

Summary

This summary is machine-generated.

Laser ablation sampling effectively captures proteins from rat brain tissue for mass spectrometry. This method quantifies proteins and enables bottom-up proteomics analysis of ablated tissue sections.

Keywords:

infrared laser laser ablation microsampling proteomics reflective objective

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Proteomics

Background:

Laser ablation sampling is a technique used for material analysis.
Mass spectrometry is a powerful tool for identifying and quantifying molecules.

Purpose of the Study:

To evaluate the effectiveness of laser ablation sampling for tissue analysis.
To quantify captured proteins from ablated tissue sections.
To prepare ablated tissue for bottom-up proteomics.

Main Methods:

Utilized a Schwarzschild reflective objective for laser ablation.
Ablated rat brain tissue sections and captured material in solvent.
Quantified proteins using a Bradford assay.
Performed liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide and protein identification.

Main Results:

Approximately 300 ng of protein was captured per 1 mm² of ablated tissue.
Demonstrated successful protein quantification and identification from ablated samples.
Established a method for bottom-up proteomics using laser-ablated tissue.

Conclusions:

Laser ablation sampling is a viable method for preparing tissue for proteomic analysis.
The technique allows for efficient capture and quantification of proteins.
This approach facilitates detailed peptide and protein identification via LC-MS/MS.