Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemiluminescence microscopy single cell imaging using esterase triggered 1,2-dioxetanes.

The Analyst·2026

Same author

Lithographic crystallinity regulation in additive fabrication of thermoplastics (CRAFT).

Science (New York, N.Y.)·2026

Same author

Harnessing Photoenzymatic Systems for Intermolecular C-H Fluorination.

Angewandte Chemie (International ed. in English)·2025

Same author

Phosphine and Arsine MOFs with Stabilized Diosmium(I) Carbonyl Sawhorse Pillars.

Inorganic chemistry·2025

Same author

One-Vat Multimaterial 3D Printing: The Devil is in the Details.

ACS central science·2025

Same author

Optimizing Green Light Photoredox Catalyzed Polymerizations for 3D Printing of Cell-Laden Hydrogels.

Biomacromolecules·2025

Same journal

From Fundamental Photophysics to Photocatalysis: Energy Gap Law Analysis of Anion Radical Excited States.

ACS central science·2026

Same journal

Mechanical Taming of Hardy-Cope Rearrangements.

ACS central science·2026

Same journal

Validation of <i>De Novo</i> Designs of Solid-Binding Peptides.

ACS central science·2026

Same journal

These Graphene Experts Are Trying to Close the Reproducibility Gap in Two-Dimensional Materials Research.

ACS central science·2026

Same journal

How to Make a Creamy, Tasty Vegan Camembert.

ACS central science·2026

Same journal

Versatile Pyridinium Trifluoroborate Platform for Facile Preparation of <sup>18</sup>F‑Labeled PET Tracers in Water.

ACS central science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 4, 2025

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Visible Light Chemical Micropatterning Using a Digital Light Processing Fluorescence Microscope.

Uroob Haris¹, Joshua T Plank¹, Bo Li¹

¹Department of Chemistry, Southern Methodist University, Dallas, Texas 75205-0314, United States.

ACS Central Science

|February 2, 2022

Summary

This summary is machine-generated.

This study introduces a digital light processing microscope for precise chemical patterning using visible light. The versatile platform enables high-resolution photochemical patterning and imaging for applications in nanorobotics and 3D printing.

More Related Videos

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Related Experiment Videos

Last Updated: Oct 4, 2025

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Area of Science:

Chemical Engineering
Materials Science
Biotechnology

Background:

Precise control over chemical reactivity is crucial for advanced technologies like nanorobotics and bioprinting.
Existing chemical patterning methods are often complex, expensive, and lack versatility.

Purpose of the Study:

To develop an accessible and versatile platform for high-resolution photochemical patterning using visible light.
To demonstrate the compatibility of the system with various visible-light-driven chemical reactions.

Main Methods:

Development of a digital light processing (DLP) fluorescence microscope.
Utilizing visible light (465-625 nm) for photochemical patterning.
Demonstration with thiol-ene reactions, Wolff rearrangements, and photopolymerizations.

Main Results:

Achieved high-resolution patterning (2.1 μm for Wolff rearrangement, 5 μm for thiol-ene).
Demonstrated photoactivation in living cells with single-cell resolution.
Enabled microscale 3D printing with 20 μm xy-resolution and 100 μm z-resolution.

Conclusions:

The DLP fluorescence microscope offers a powerful, user-friendly platform for advanced chemical patterning.
This technology facilitates the development of next-generation nanorobotics, 3D printing, and metamaterials.