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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
Published on: May 21, 2019
MicroRNA-30c-2-3p targets STRIP2 to suppress malignant progression of gastric cancer cells
Junfei Wu1, Guochun Lu1, Shengkun Zhou1
1Department of General Surgery, First People's Hospital of Tonglu, Tonglu 311500, China.
Abstract:
MicroRNA plays a crucial part in genesis and development of gastric cancer (GC). We uncovered that microRNA-30c-2-3p was down-regulated in GC tissue and cell lines. Suppression of microRNA-30c-2-3p promoted progression of GC cells in vitro. STRIP2 was confirmed as a target for microRNA-30c-2-3p. MicroRNA-30c-2-3p overexpression remarkably suppressed cell malignant behaviours, while reintroduction of STRIP2 partially restored the anticancer effect of microRNA-30c-2-3p. Taken together, these findings suggested that microRNA-30c-2-3p acted as a candidate tumour suppressor in GC by directly targeting STRIP2. Therefore, microRNA-30c-2-3p can be used as a towardly GC therapeutic target.
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