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Invasive Escherichia coli.

S B Formal, R B Hornick

    The Journal of Infectious Diseases
    |May 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Transferring Shigella pathogenicity to E. coli K12 via genetic recombination has been unsuccessful. Multiple genes control Shigella-like virulence, making random DNA insertion unlikely to confer invasive enteric pathogen traits.

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    Area of Science:

    • Microbiology and Genetics
    • Bacterial Pathogenesis
    • Molecular Biology

    Background:

    • Shigella pathogenicity is a complex trait governed by numerous genes.
    • Escherichia coli (E. coli) strain K12 is a common laboratory strain lacking invasive virulence.
    • Conferring invasive virulence to E. coli K12 is a long-standing research goal.

    Purpose of the Study:

    • To investigate the feasibility of transferring Shigella-like pathogenicity to E. coli K12.
    • To assess the effectiveness of classical recombination procedures for creating virulent E. coli K12.
    • To understand the genetic basis of invasive enteric pathogenicity.

    Main Methods:

    • Classical genetic recombination techniques were employed.
    • Donor strains of virulent Shigella flexneri were used.

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  • Attempts were made to create hybrid E. coli K12 clones with acquired virulence.

    • Main Results:

    • Attempts to confer invasive virulence on E. coli K12 using recombination with S. flexneri were unsuccessful.
    • The complexity of screening for pathogenicity in numerous hybrid clones presented a significant practical challenge.
    • Invasive pathogenicity is determined by multiple genetic loci, not easily replicated by random insertion.

    Conclusions:

    • Transferring Shigella-like pathogenicity to E. coli K12 through classical recombination is currently unachieved.
    • The polygenic nature of invasive virulence makes random foreign DNA insertion an improbable method for creating virulent E. coli K12.
    • Further research into the specific genetic determinants of Shigella pathogenicity is warranted.