Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gene-Specific Endothelial Programs Drive AVM Pathogenesis in <i>SMAD4</i> and <i>ALK1</i> Loss-of-Function.

Arteriosclerosis, thrombosis, and vascular biology·2026

Same author

Investigating the determinants of immunotherapy response in the primary tumour of clear cell renal cell carcinoma (RCC).

BMJ oncology·2026

Same author

CpG Atlas: A centralized multi-layer database and AI interface for DNA methylation research.

bioRxiv : the preprint server for biology·2026

Same author

Plasminogen Activator Inhibitor-1 is Internalized by Endothelial Cells via A Pinocytosis-Like Mechanism and Evades Degradation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Deletion of endothelial KLF4 as a model for preeclampsia.

bioRxiv : the preprint server for biology·2026

Same author

PAD-associated Genetic Variants are More Strongly Associated with Surgical Intervention than Premature Onset.

Journal of cardiovascular translational research·2026

Search research articles

Related Experiment Video

Updated: Oct 4, 2025

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Prdm6 controls heart development by regulating neural crest cell differentiation and migration.

Lingjuan Hong¹, Na Li¹, Victor Gasque¹

¹Cardiovascular Research Center, Department of Internal Medicine.

|February 2, 2022

Summary

Prdm6 is an epigenetic modifier crucial for cardiac neural crest cell (CNCC) development. Its disruption impairs CNCC fate, migration, and leads to congenital heart defects, highlighting Prdm6 as a potential therapeutic target.

Keywords:

Cardiology Cardiovascular disease Development Embryonic development Epigenetics

More Related Videos

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Published on: May 24, 2020

Related Experiment Videos

Last Updated: Oct 4, 2025

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Published on: February 7, 2012

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Published on: May 24, 2020

Area of Science:

Developmental biology
Epigenetics
Cardiovascular research

Background:

The molecular basis for neural crest cell (NCC) fate determination remains incompletely understood.
Cardiac NCCs (CNCCs) are vital for heart development, and their proper differentiation, delamination, and migration are essential for preventing congenital heart disease.

Purpose of the Study:

To identify key regulators of CNCC fate and migration.
To elucidate the role of the epigenetic modifier Prdm6 in CNCC development.
To explore Prdm6 as a potential therapeutic target for congenital heart diseases.

Main Methods:

Transcriptomic analysis (bulk and single-cell RNA-Seq) in transgenic mice.
Genetic manipulation and fate mapping of CNCCs.
Analysis of epigenetic modifications (H4K20 monomethylation) and cell cycle progression.

Main Results:

Prdm6 regulates the expression of critical CNCC specifier genes, including Wnt1, Tfap2b, and Sox9.
Loss of Prdm6 disrupts CNCC differentiation, delamination, and migration, leading to patent ductus arteriosus and ventricular noncompaction.
Prdm6 deficiency causes retention of NCCs in the dorsal neural tube due to impaired epithelial-mesenchymal transition, linked to altered H4K20 monomethylation and Wnt1 expression.

Conclusions:

Prdm6 is a critical epigenetic regulator of CNCC differentiation and migration.
Prdm6 and its downstream gene network represent promising targets for treating congenital heart diseases.