Direct Contact with Platelets Induces Podoplanin Expression and Invasion in Human Oral Squamous Cell Carcinoma Cells
Se-Young Park1,2,3, Sun Kyoung Lee3, Mihwa Lim3
1Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
Biomolecules & Therapeutics
|February 3, 2022
Summary
Platelets enhance oral cancer metastasis by increasing PDPN expression in cancer cells. Targeting podoplanin (PDPN) offers a novel therapeutic strategy for oral squamous cell carcinoma (OSCC) treatment, avoiding risks associated with traditional antiplatelet drugs.
Area of Science:
- Oncology
- Hematology
- Cell Biology
Background:
- Oral squamous cell carcinoma (OSCC) often presents with advanced metastasis, necessitating effective treatment strategies.
- Platelets are implicated in cancer metastasis, but their role in modulating PDPN expression in OSCC is unclear.
Purpose of the Study:
- To investigate the reciprocal effect of platelets on podoplanin (PDPN) expression in oral squamous cell carcinoma (OSCC).
- To explore PDPN as a potential therapeutic target for OSCC metastasis.
Main Methods:
- Investigated platelet-tumor cell interactions using human OSCC cell lines (Ca9.22 and HSC3).
- Assessed PDPN and integrin β1 expression via Western blotting and cell invasiveness assays.
- Analyzed public databases for PDPN and integrin β1 co-expression in oral tissues.
Main Results:
- Direct platelet contact upregulated PDPN and integrin β1 in low- PDPN OSCC cells, promoting invasiveness.
- Silencing PDPN in high-PDPN OSCC cells reduced invasiveness.
- Co-expression of PDPN and integrin β1 was observed, with higher levels in metastatic tissues.
Conclusions:
- Platelet interaction with OSCC cells upregulates PDPN, enhancing metastasis.
- PDPN is a potential therapeutic target for OSCC, offering an alternative to traditional antiplatelet agents.
- Targeting PDPN may overcome limitations of current antiplatelet therapies in managing OSCC metastasis.
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