TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom.

Area of Science:

• Biochemistry
• Peptide Science
• Toxicology

Background:

• Anionic peptides from scorpion venom are underexplored and possess unique properties.
• TanP, a linear anionic peptide from *Tityus stigmurus*, exhibits Cu2+ chelation and immunomodulatory effects.
• Chelating molecules have therapeutic applications in metal intoxication, neurodegenerative diseases, and cancer.

Purpose of the Study:

• To evaluate TanP's chelating activity for Fe2+ and Zn2+.
• To investigate TanP's antioxidant, hemostatic, immunomodulatory, and wound healing potential.
• To explore the biotechnological and therapeutic applications of TanP.

Main Methods:

• Spectrophotometric analysis to assess Fe2+ and Zn2+ complexation.
• Hemolysis assay for biocompatibility.
• DPPH and hydroxyl radical scavenging assays for antioxidant activity.
• Prothrombin time and aPTT assays for anticoagulant activity.
• TNF-α release assay and in vitro wound healing assay.

Main Results:

• TanP forms stable complexes with Fe2+ (1:5 ratio) and acts as a potential Fe2+ sensor.
• TanP exhibits significant antioxidant activity, including 89.7% iron chelation and high radical scavenging.
• TanP displays anticoagulant effects and stimulates TNF-α release and cell migration, indicating healing potential.

Conclusions:

• TanP is a multifunctional peptide with promising therapeutic and biotechnological applications.
• Its iron-chelating and antioxidant properties are notable.
• TanP's immunomodulatory and wound healing capabilities warrant further investigation for drug development.