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Related Experiment Video

Updated: Oct 4, 2025

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

SARS-CoV-2 Genome Sequence Obtained from Ethiopia.

Molalegne Bitew¹, Getnet Hailu², Yakob Gebregziabher Tsegay³

¹Ethiopia Biotechnology Institute (EBTI), Addis Ababa, Ethiopia.

Microbiology Resource Announcements

|February 3, 2022

Summary

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome sequencing in Ethiopia revealed the B.1 lineage as dominant. The concerning B.1.1.7 variant also emerged during the study period.

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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

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Area of Science:

Virology
Genomics
Epidemiology

Background:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic.
Understanding viral lineages circulating in different regions is crucial for public health.
Ethiopia's SARS-CoV-2 genomic surveillance data was limited.

Purpose of the Study:

To determine the SARS-CoV-2 genome sequences from samples collected in Addis Ababa, Ethiopia.
To identify the prevalent SARS-CoV-2 lineages and track their emergence.

Main Methods:

Whole-genome sequencing of 15 nasopharyngeal swab samples using Illumina MiSeq technology.
Sequence analysis to identify SARS-CoV-2 lineages.

Main Results:

The B.1 lineage of SARS-CoV-2 was found to be the most prevalent.
Emergence of the B.1.1.7 variant was detected in June 2021.

Conclusions:

The B.1 lineage was the dominant SARS-CoV-2 strain in Addis Ababa between December 2020 and March 2021.
The emergence of the B.1.1.7 variant in Ethiopia highlights the need for continued genomic surveillance.