Related Experiment Video

Updated: Oct 4, 2025

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.9K

Tumour-suppressing functions of the lncRNA MBNL1-AS1/miR-889-3p/KLF9 axis in human breast cancer cells

Yongmei Jin1, Lingli Xu2, Bin Zhao2

  • 1Department of Nursing, Shanghai Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Cell Cycle (Georgetown, Tex.)
|February 3, 2022
PubMed

Insights

The long non-coding RNA MBNL1-AS1 suppresses breast cancer progression by inhibiting cell proliferation, invasion, migration, and glycolysis. MBNL1-AS1 acts via the miR-889-3p/KLF9 pathway, suggesting its potential as a diagnostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of mortality in women worldwide.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of MBNL1-AS1 in breast cancer remains largely unexplored.

Purpose of the Study:

  • To investigate the role and underlying mechanism of lncRNA MBNL1-AS1 in human breast cancer.
  • To determine the relationship between MBNL1-AS1 expression and clinical prognosis.
  • To elucidate the molecular interactions involving MBNL1-AS1, miR-889-3p, and KLF9.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting to assess gene and protein expression.
  • Cell-based assays to evaluate proliferation, invasion, migration, and glycolysis.
  • Luciferase reporter and pull-down assays to confirm molecular interactions.
  • Transfection of MBNL1-AS1, miR-889-3p mimics, and si-KLF9 in breast cancer cell lines.

Main Results:

  • MBNL1-AS1 expression was significantly downregulated in breast cancer tissues and cell lines.
  • Low MBNL1-AS1 levels correlated with poor patient prognosis.
  • Overexpression of MBNL1-AS1 inhibited breast cancer cell proliferation, invasion, migration, and glycolysis.
  • MBNL1-AS1 directly targets miR-889-3p, which in turn targets KLF9, forming an inhibitory axis.

Conclusions:

  • lncRNA MBNL1-AS1 functions as a tumor suppressor in breast cancer.
  • The MBNL1-AS1/miR-889-3p/KLF9 axis plays a critical role in regulating breast cancer cell behaviors.
  • MBNL1-AS1 holds potential as a diagnostic biomarker and therapeutic target for breast cancer.
Keywords:
Breast cancerKLF9lncRNA MBNL1-AS1miR-889-3p

More Related Videos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.6K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.3K