Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin protects against hyperoxic decrease in membrane fluidity in endothelial cells but not in fibroblasts.

E R Block, J M Patel, N P Sheridan

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |February 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Five-year survival with tebentafusp in metastatic uveal melanoma.

    Annals of oncology : official journal of the European Society for Medical Oncology·2026
    Same author

    Cardiovascular subphenotypes in patients with COVID-19 pneumonitis whose lungs are mechanically ventilated: a single-centre retrospective observational study.

    Anaesthesia·2022
    Same author

    Ionization of Hydrofluoric Acid at 25 °C.

    Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2021
    Same author

    Marked differences in local bone remodelling in response to different marrow stimulation techniques in a large animal.

    European cells & materials·2021
    Same author

    Renal impairment and its impact on clinical outcomes in patients who are critically ill with COVID-19: a multicentre observational study.

    Anaesthesia·2021
    Same author

    Association of clinical factors and recent anticancer therapy with COVID-19 severity among patients with cancer: a report from the COVID-19 and Cancer Consortium.

    Annals of oncology : official journal of the European Society for Medical Oncology·2021
    Same journal

    Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Transcription Factor Ets-1 is a Central Regulator of Redox Balance and Liver Regeneration Through EGF and TGF-β1 Signaling.

    Laboratory investigation; a journal of technical methods and pathology·2026
    See all related articles

    Endotoxin protects endothelial cells from hyperoxia-induced membrane damage by maintaining plasma membrane fluidity. This protective effect was not observed in fibroblasts, suggesting cell-specific mechanisms against oxidative stress.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Physiology

    Background:

    • Hyperoxia can depress plasma membrane fluidity, potentially leading to cellular injury.
    • Endotoxin's protective effects against cellular stress are not fully understood.
    • Endothelial cells and fibroblasts are relevant models for studying cellular responses to oxidative stress.

    Purpose of the Study:

    • To evaluate endotoxin's ability to protect against hyperoxic depression of plasma membrane fluidity in cultured endothelial cells and fibroblasts.
    • To investigate the cell-specific effects of endotoxin on membrane fluidity under hyperoxic conditions.

    Main Methods:

    • Porcine aortic endothelial cells and human fibroblasts were cultured with or without endotoxin.
    • Cells were exposed to normoxia (20% O2) or hyperoxia (95% O2) for 4 hours.

    Related Experiment Videos

  • Plasma membrane fluidity was assessed using 1,6-diphenyl-1,3,5-hexatriene (DPH) and transparinaric acid (TPA) with fluorescence spectroscopy.

    • Main Results:

    • Hyperoxia increased membrane rigidity (reduced fluidity) in both endothelial cells and fibroblasts, indicated by increased fluorescence anisotropy (rs).
    • Endotoxin prevented the hyperoxia-induced increase in membrane rigidity in endothelial cells.
    • Endotoxin did not prevent the hyperoxia-induced increase in membrane rigidity in fibroblasts.

    Conclusions:

    • Hyperoxia decreases plasma membrane fluidity in both endothelial cells and fibroblasts.
    • Endotoxin specifically protects endothelial cells, but not fibroblasts, from hyperoxia-induced decreases in plasma membrane fluidity.
    • Endotoxin's membrane-protective effects in endothelial cells may represent a novel mechanism against hyperoxic cellular injury.