Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

4.1K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.1K
Protein-protein Interfaces02:04

Protein-protein Interfaces

13.9K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.9K
Proteomics01:33

Proteomics

8.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leaf Fungal Microbiome Is Modulated by Interspecific Hybridization Events Between Coffea Species.

Physiologia plantarum·2026
Same author

Metagenome-assembled genomes of four novel bacterial species from Atlantic rainforest stream sediments in Brazil.

Microbiology resource announcements·2026
Same author

Addressing Vitamin B<sub>12</sub> deficiency through aeroponic fortification of a salad crop (Pisum sativum).

Communications biology·2026
Same author

First report of the coffee leaf miner (Leucoptera coffeella) bacteriome in larval/adult stages and possible contribution of the plant host.

FEMS microbiology letters·2025
Same author

Circadian and environmental signal integration in a natural population of <i>Arabidopsis</i>.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Circadian regulation of metabolism across photosynthetic organisms.

The Plant journal : for cell and molecular biology·2023

Related Experiment Video

Updated: Oct 4, 2025

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

16.4K

Interactomes: Experimental and In Silico Approaches.

Luíza Lane de Barros Dantas1, Marcelo Mendes Brandão2

  • 1John Innes Centre, Norwich Research Park, Norwich, UK. luiza.bd@gmail.com.

Advances in Experimental Medicine and Biology
|February 3, 2022
PubMed
Summary

Understanding protein-protein interactions (PPIs) is crucial for molecular biology processes like DNA replication, transcription, and translation. This chapter explores biochemical and computational methods to study these essential protein interactions and fill gaps in interactome data.

Keywords:
BioinformaticsDatabasesInteractomeProtein–Protein interactionsProteome

More Related Videos

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.3K
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

13.5K

Related Experiment Videos

Last Updated: Oct 4, 2025

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

16.4K
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.3K
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

13.5K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Computational Biology

Background:

  • Protein-protein interactions (PPIs) are fundamental to all aspects of the Central Dogma of Molecular Biology.
  • Processes such as DNA replication, transcription, and translation rely heavily on intricate PPIs.
  • Understanding these interactions is key to deciphering cellular mechanisms.

Purpose of the Study:

  • To explore techniques for constructing and completing interactome studies.
  • To address gaps in current knowledge of protein interactions.
  • To provide a comprehensive overview of PPI assessment methodologies.

Main Methods:

  • Direct assessment of biochemical and molecular machinery involved in protein interactions.
  • Utilizing computational biology techniques to gather PPI information.
  • Leveraging public databases for interactome data analysis.

Main Results:

  • The chapter details methods to investigate how proteins interact.
  • It outlines strategies to fill missing information in protein interaction networks.
  • Both experimental and computational approaches are presented for interactome analysis.

Conclusions:

  • A robust understanding of PPIs requires diverse methodologies.
  • Combining biochemical and computational approaches enhances interactome study completeness.
  • This work provides a framework for advancing PPI research.