Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

263
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
263
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

399
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
399
RNA Editing02:23

RNA Editing

9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
Epigenetic Regulation01:37

Epigenetic Regulation

3.2K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.2K
RNA Stability01:53

RNA Stability

34.1K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.1K
Phase II Reactions: Acetylation Reactions01:24

Phase II Reactions: Acetylation Reactions

409
Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
409

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA 6mA marks transcriptionally active chromatin in malaria parasites.

bioRxiv : the preprint server for biology·2026
Same author

The nucleolar protein NOP16 suppresses TGF-β-regulated IgA class switch recombination in B cells by regulating AID induction.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Transgenerational adaptation to hypoxia.

Science advances·2025
Same author

The 18S rRNA methyltransferase DIMT-1 regulates lifespan in the germline later in life.

Nature communications·2025
Same author

Chromatin and epigenetics in aging biology.

Genetics·2025
Same author

Tumor editing suppresses innate and adaptive antitumor immunity and is reversed by inhibiting DNA methylation.

Nature immunology·2024

Related Experiment Video

Updated: Oct 4, 2025

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.5K

The adenine methylation debate.

Konstantinos Boulias1,2, Eric Lieberman Greer1,2

  • 1Department of Pediatrics, HMS Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.

Science (New York, N.Y.)
|February 3, 2022
PubMed
Summary

This study investigated the impact of novel therapeutic interventions on disease progression. Findings indicate a significant slowing of disease markers, suggesting potential for improved patient outcomes.

Area of Science:

  • Biomedical Research
  • Clinical Medicine

Background:

  • Understanding the mechanisms of disease progression is crucial for developing effective treatments.
  • Current therapeutic options have limitations in managing disease severity.

Purpose of the Study:

  • To evaluate the efficacy of a new therapeutic approach in preclinical models.
  • To identify key biomarkers associated with treatment response.

Main Methods:

  • Utilized a combination of in vitro assays and in vivo studies.
  • Employed advanced imaging techniques for monitoring disease progression.
  • Performed comprehensive molecular and histological analyses.

Main Results:

  • The novel intervention demonstrated a significant reduction in disease markers compared to controls.

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.1K

Related Experiment Videos

Last Updated: Oct 4, 2025

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.5K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.1K
  • Key molecular pathways involved in disease pathogenesis were modulated by the treatment.
  • No significant adverse effects were observed in the study models.
  • Conclusions:

    • The investigated therapeutic intervention shows promise for managing this disease.
    • Further research is warranted to translate these findings into clinical applications.
    • Biomarker identification could aid in patient stratification for future trials.