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Updated: Oct 4, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Long non-coding RNA DLGAP1 antisense RNA 1 accelerates glioma progression via the microRNA-628-5p/DEAD-box helicase

Ke-Qi Hu1, Xiang-Sheng Ao1

  • 1Department of Neurosurgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, China.

Clinics (Sao Paulo, Brazil)
|February 3, 2022
PubMed
Abstract

Insights

Long non-coding RNA DLGAP1-AS1 promotes glioma progression by sponging miR-628-5p, leading to increased DDX59 expression. This axis is a potential therapeutic target for glioma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Abnormal expression of long non-coding RNAs (lncRNAs) is implicated in glioma progression.
  • The specific role of lncRNA DLGAP1 antisense RNA 1 (DLGAP1-AS1) in glioma remains largely uncharacterized.

Purpose of the Study:

  • To investigate the biological function and mechanism of DLGAP1-AS1 in glioma.
  • To elucidate the regulatory pathway involving DLGAP1-AS1, miR-628-5p, and DDX59 in glioma.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) assays (CCK-8, EdU, Transwell, Western blot).
  • Dual-luciferase reporter gene assay to confirm molecular interactions.

Main Results:

  • DLGAP1-AS1 expression is upregulated in higher-grade gliomas and correlates with shorter survival.
  • DLGAP1-AS1 enhances glioma cell proliferation, migration, invasion, and EMT.
  • DLGAP1-AS1 acts as a molecular sponge for miR-628-5p, and this interaction upregulates DEAD-box helicase 59 (DDX59) expression.

Conclusions:

  • The DLGAP1-AS1/miR-628-5p/DDX59 axis is a key regulator of glioma progression.
  • DLGAP1-AS1 promotes glioma development, partly through the miR-628-5p/DDX59 pathway.
  • Targeting this axis may offer a novel therapeutic strategy for glioma.
Keywords:
DDX59DLGAP1-AS1GliomamiR-628-5p

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