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A microfluidic biosensor for rapid simultaneous detection of waterborne pathogens.

Sura A Muhsin1, Muthana Al-Amidie2, Zhenyu Shen3

  • 1Department of Electrical and Computer Engineering, University of Missouri, 411 S. 6th St., room 201, Columbia, MO, 65211, USA.

Biosensors & Bioelectronics
|February 3, 2022
PubMed
Summary

This study presents a microfluidic biosensor for rapid, simultaneous detection of Salmonella, Legionella, and E. coli O157:H7. The device achieves a low detection limit, offering a quick solution for water quality monitoring.

Keywords:
AntibodiesBacterial cellsBiosensorDielectrophoresisImpedanceSimultaneous detection

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Area of Science:

  • Biomedical Engineering
  • Environmental Science
  • Microfluidics

Background:

  • Accurate and rapid detection of waterborne pathogens is crucial for public health.
  • Existing methods for detecting multiple bacteria like Salmonella, Legionella, and E. coli O157:H7 can be time-consuming and complex.
  • There is a need for sensitive, simultaneous detection platforms for diverse bacterial contaminants in water.

Purpose of the Study:

  • To develop and validate a microfluidic biosensor for the simultaneous detection of three key bacterial pathogens.
  • To assess the biosensor's performance in detecting Salmonella, Legionella, and Escherichia coli O157:H7 in various water matrices.
  • To achieve rapid detection with a high degree of sensitivity and specificity.

Main Methods:

  • Fabrication of a microfluidic chip with focusing and interdigitated electrode (IDE) arrays.
  • Utilizing positive dielectrophoresis (p-DEP) for bacterial concentration at the microchannel centerline.
  • Immobilization of specific antibodies onto IDEs for selective capture and impedance-based detection.

Main Results:

  • Simultaneous detection of Salmonella, Legionella, and E. coli O157:H7 was achieved.
  • The biosensor demonstrated a low limit of detection of 3 bacterial cells/ml.
  • Detection was completed within a rapid timeframe of 30-40 minutes.

Conclusions:

  • The developed microfluidic biosensor offers a rapid and sensitive platform for simultaneous detection of multiple waterborne pathogens.
  • This technology has significant potential for real-time water quality monitoring and public health protection.
  • The biosensor's ability to concentrate bacteria enhances detection limits for environmental and clinical applications.