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Long non-coding RNA DLX6-AS1 knockdown suppresses the tumorigenesis and progression of non-small cell lung cancer

Chengde Wu1, Wei Lin1, Fangyong Fu1

  • 1Department of Thoracic Surgery, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou People's Hospital, Haidian Island, Haikou, China.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

Downregulating long non-coding RNA DLX6-AS1 inhibits non-small cell lung cancer (NSCLC) progression by targeting the microRNA-16-5p/BMI1 pathway. This finding offers a potential therapeutic strategy for NSCLC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) poses a significant health burden.
  • Long non-coding RNA (lncRNA) DLX6-AS1 is implicated in various cancers.
  • The specific role of DLX6-AS1 in NSCLC tumorigenesis requires elucidation.

Purpose of the Study:

  • To investigate the role and underlying mechanism of DLX6-AS1 in NSCLC.
  • To determine the relationship between DLX6-AS1, microRNA-16-5p (miR-16-5p), and BMI1 in NSCLC.

Main Methods:

  • Quantitative PCR (qPCR) and Western blot for molecular level analysis.
  • Cell proliferation, migration, invasion, and apoptosis assays for functional characterization.
  • Bioinformatics, luciferase reporter, and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.
  • In vivo xenograft mouse model to assess DLX6-AS1's effect on tumor growth.

Main Results:

  • DLX6-AS1 was upregulated in NSCLC tissues and correlated with poor prognosis.
  • DLX6-AS1 depletion suppressed NSCLC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while promoting apoptosis.
  • DLX6-AS1 acts as a sponge for miR-16-5p, which directly targets BMI1.
  • DLX6-AS1 silencing inhibited tumor growth in vivo.

Conclusions:

  • lncRNA DLX6-AS1 downregulation suppresses NSCLC tumorigenesis and progression.
  • The miR-16-5p/BMI1 axis is a key downstream pathway regulated by DLX6-AS1 in NSCLC.
  • DLX6-AS1 is a potential therapeutic target for NSCLC.
Keywords:
BMI1Long non-coding RNA distal-less homeobox 6 antisense RNA 1 (lncRNA DLX6-AS1)microRNA-16-5p (miR-16-5p)non-small cell lung cancer (NSCLC)

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