Related Experiment Video
Updated: Oct 4, 2025
12:14
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
Published on: June 2, 2022
2.8K
Nav1.5-E3 antibody inhibits cancer progression
Rui Gao1, Ting Cao1, Hui Chen1
1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Translational Cancer Research
|February 4, 2022
Summary
Nav1.5, a sodium channel overexpressed in cancer, was targeted by E3Ab. This antibody inhibited cancer cell migration, invasion, and tumor growth in vivo, suggesting E3Ab as a potential cancer therapy.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Voltage-gated sodium channel Nav1.5 is overexpressed in cancer cells, correlating with disease progression.
- Nav1.5-third extracellular region antibody (E3Ab) specifically targets Nav1.5, inhibiting its function.
- The therapeutic potential of E3Ab in cancer treatment warrants investigation.
Purpose of the Study:
- To evaluate the therapeutic potential of E3Ab in preclinical cancer models.
- To assess the effect of E3Ab on cancer cell behavior and tumor growth.
Main Methods:
- Nav1.5 expression was confirmed in Caov-3 cells via immunocytochemistry.
- In vivo tumor growth was assessed using a Caov-3 xenograft nude mouse model.
- In vitro assays evaluated the impact of E3Ab on cancer cell migration, invasion, and proliferation, with MMP-9 expression analyzed by Western blot.
Main Results:
- Caov-3 cells exhibited high Nav1.5 expression, with confirmed binding by E3Ab.
- E3Ab and lidocaine significantly inhibited Caov-3 xenograft tumor growth and reduced mitotic activity in vivo.
- In vitro, E3Ab and lidocaine suppressed cancer cell migration and invasion.
Conclusions:
- E3Ab demonstrates significant anti-cancer effects by inhibiting tumor growth, migration, and invasion.
- These findings highlight E3Ab as a promising therapeutic antibody for cancers expressing Nav1.5.
Related Concept Videos
02:38Mitogens and the Cell Cycle
7.0K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.0K
02:07Tumor Progression
6.6K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.6K
02:57Targeted Cancer Therapies
7.9K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.9K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
02:23Abnormal Proliferation
4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
02:53Adaptive Mechanisms in Cancer Cells
6.0K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.0K