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Related Experiment Video

Updated: Oct 4, 2025

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

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GEFT aberrant expression in soft tissue sarcomas.

Yang Liu¹, Shengnan Qi¹, Lian Meng¹

¹Department of Pathology, Shihezi University School of Medicine, Shihezi 832002, China.

Translational Cancer Research

|February 4, 2022

Summary

Guanine nucleotide exchange factor T (GEFT) is abnormally expressed in most soft tissue sarcomas (STS). This aberrant GEFT expression, observed across various STS subtypes, may contribute to tumor development.

Keywords:

Guanine nucleotide exchange factor T (GEFT)chromosomal translocation immunohistochemistry non-chromosomal translocation-associated soft tissue sarcoma (STS)

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Published on: January 22, 2018

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Guanine nucleotide exchange factor T (GEFT) shows high amplification in rhabdomyosarcoma.
GEFT protein is overexpressed in rhabdomyosarcoma compared to normal striated muscle.
This study investigates GEFT expression in various soft tissue sarcomas (STS).

Purpose of the Study:

Evaluate aberrant GEFT expression in multiple STS subtypes.
Compare GEFT expression (protein and mRNA) between chromosomal translocation-associated sarcomas (CTAS) and non-chromosomal translocation-associated sarcomas (NCTAS).
Analyze differences in clinical pathology and histological features related to GEFT expression.

Main Methods:

Immunohistochemistry (IHC) and tissue microarrays for GEFT protein detection.
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for GEFT mRNA expression analysis.

Main Results:

GEFT positive expression (89.50%) and overexpression (51.60%) were significantly higher in STS than in normal striated muscle.
Aberrant GEFT expression was observed across all evaluated STS subtypes.
No significant difference in GEFT expression was found between CTAS and NCTAS.

Conclusions:

Abnormal GEFT expression is prevalent in diverse STS subtypes.
GEFT may play a crucial role in the tumorigenesis of soft tissue sarcomas.