Microarray-based differential expression profiling of long noncoding RNAs and messenger RNAs in formalin-fixed...

Area of Science:

• Molecular Biology
• Genomics
• Oncology

Background:

• Long noncoding RNAs (lncRNAs) regulate gene expression and are implicated in various cancers.
• The role of lncRNAs in papillary thyroid carcinoma (PTC) remains largely unexplored.

Purpose of the Study:

• To identify novel lncRNAs involved in the development of papillary thyroid carcinoma (PTC).
• To explore the potential diagnostic and therapeutic implications of these lncRNAs in PTC.

Main Methods:

• Microarray analysis of mRNA and lncRNA expression in PTC and nodular goiter tissues.
• Quantitative polymerase chain reaction (q-PCR) for validation of differentially expressed genes.
• Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
• Co-expression analysis to identify regulatory relationships between lncRNAs and target genes.

Main Results:

• Aberrant expression of 94 lncRNAs and 99 mRNAs was observed in PTC tissues compared to nodular goiter.
• Microarray findings were validated using q-PCR.
• Co-expression analysis highlighted the association of lncRNAs n382996, n342483, and n409114 with the regulation of MT1G, MT1H, and MT1F.

Conclusions:

• Novel lncRNAs associated with PTC have been identified.
• These findings suggest potential roles for specific lncRNAs in PTC pathogenesis.
• Further investigation is warranted to explore the diagnostic and therapeutic potential of these novel lncRNAs in PTC.