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Updated: Oct 4, 2025

Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors
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microRNA-324 plays an oncogenic role in bladder cancer cell growth and motility

Kuo-Wang Tsai1,2,3, Wei-Ting Kuo4,5,6, Shaw-Yeu Jeng4,5

  • 1Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

High levels of miR-324 microRNA (miRNA) are linked to poor bladder cancer survival. This study shows miR-324 acts as an oncogene, promoting cancer cell growth and invasion, suggesting it could be a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Late-stage bladder cancer (BC) has a high mortality rate despite treatment advances.
  • Dysfunctional microRNA (miRNA) expression is linked to cancer prognosis.
  • The specific role of miR-324 in BC pathogenesis was previously unclear.

Purpose of the Study:

  • To investigate the expression levels and biological roles of miR-324-5p and miR-324-3p in bladder cancer.
  • To determine the correlation between miR-324 expression and patient survival.
  • To explore the potential of miR-324 as a therapeutic target or biomarker for BC.

Main Methods:

  • Analyzed miR-324-5p and miR-324-3p expression using The Cancer Genome Atlas (TCGA) database and real-time PCR.
  • Assessed the functional impact of miR-324-5p and miR-324-3p in BFTC950 cells via mimic transfection.

Main Results:

  • Elevated miR-324-5p and miR-324-3p expression significantly correlated with poorer patient survival in BC.
  • miR-324-5p significantly enhanced BC cell proliferation, colony formation, and invasion.
  • miR-324-3p showed a slight increase in BC cell growth and motility.

Conclusions:

  • miR-324-5p and miR-324-3p function as oncogenes in bladder cancer cells.
  • These findings offer novel insights into potential therapeutic strategies and biomarkers for BC.
Keywords:
Bladder cancer (BC)miR-324-3pmiR-324-5p

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