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Lung cancer A549 cells suppressed with overexpressed HNF1B or PCDHA13 inhibited PI3K/AKT phosphorylation

Chunyan Kang1, Lingxiao Wang1, Dandan Wang2

  • 1Department of Pathology, Henan Medical College, Zhengzhou 451191, China.

Translational Cancer Research
|February 4, 2022
PubMed
Abstract

Insights

Overexpressing hepatocyte nuclear factor 1B (HNF1B) and PCDHA13 in lung cancer cells reduces proliferation and invasion while increasing apoptosis. This suggests HNF1B and PCDHA13 inhibit malignant behavior by reducing PI3K/AKT phosphorylation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Lung cancer remains a leading cause of cancer-related mortality globally.
  • Investigating novel molecular targets is crucial for developing effective lung cancer therapies.
  • Hepatocyte nuclear factor 1B (HNF1B) and PCDHA13 roles in lung cancer are not fully understood.

Purpose of the Study:

  • To investigate the effects of HNF1B and PCDHA13 overexpression on PI3K/AKT signaling.
  • To determine the impact of HNF1B and PCDHA13 on the malignant biological behavior of lung cancer A549 cells.
  • To explore potential therapeutic strategies targeting HNF1B and PCDHA13 in lung cancer.

Main Methods:

  • Overexpression plasmids for HNF1B and PCDHA13 were constructed and transfected into A549 cells.
  • Quantitative reverse transcription PCR (RT-PCR) assessed mRNA levels.
  • Cell proliferation (clone formation), apoptosis (flow cytometry), and invasion (Transwell assay) were evaluated.
  • Protein expression and PI3K/AKT phosphorylation levels were analyzed via immunoblotting.

Main Results:

  • HNF1B and PCDHA13 mRNA and protein levels were significantly upregulated post-transfection.
  • Overexpression of HNF1B and PCDHA13 markedly reduced cell proliferation and invasion.
  • Apoptosis rates and cleaved caspase expression significantly increased, indicating enhanced cell death.
  • PI3K/AKT phosphorylation was significantly inhibited in cells overexpressing HNF1B or PCDHA13.

Conclusions:

  • Overexpression of HNF1B and PCDHA13 suppresses malignant phenotypes in lung cancer A549 cells.
  • HNF1B and PCDHA13 inhibit PI3K/AKT phosphorylation, a key pathway in cancer progression.
  • These findings highlight HNF1B and PCDHA13 as potential therapeutic targets for lung cancer.
Keywords:
Hepatocyte nuclear factor 1B and PCDHA13 (HNF1B and PCDHA13)PI3K/AKTapoptosisinvasionlung cancerproliferation

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