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Updated: Oct 4, 2025
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC
Haruko Watanabe-Takano1, Moe Fukumoto1, Shigetomo Fukuhara2
1Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-shimmachi, Suita, Osaka 564-8565, Japan.
Abstract:
Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining the developmental stage-specific expression of genes of interest spatially and temporally. For complete details on the use and execution of this protocol, please refer to Watanabe-Takano et al. (2021).
