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Related Experiment Video

Updated: Oct 4, 2025

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Published on: August 8, 2012

A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer.

Hisashi Kanemaru¹, Yukari Mizukami¹, Akira Kaneko¹

¹Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.

|February 4, 2022

Summary

This study introduces a label-free, impedance-based method for real-time analysis of lymphocyte-mediated antitumor cytotoxicity. This novel approach offers continuous, noninvasive monitoring, generating real-time killing curves for accurate cytotoxicity quantification.

Keywords:

Cancer Cell Biology Immunology

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Last Updated: Oct 4, 2025

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Published on: August 8, 2012

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Published on: December 27, 2016

Real-time Cytotoxicity Assays in Human Whole Blood

Real-time Cytotoxicity Assays in Human Whole Blood

Published on: November 7, 2014

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Standard assays for lymphocyte-mediated antitumor cytotoxicity rely on radioisotopic or fluorescent labels, limiting real-time analysis.
Existing methods are not suitable for continuous, noninvasive monitoring of cellular responses.

Purpose of the Study:

To introduce a novel, label-free protocol for real-time analysis of lymphocyte-mediated cytotoxicity.
To demonstrate the utility of an impedance-based cell analyzer for quantifying antitumor immune responses.

Main Methods:

Utilized a label-free, impedance-based real-time cell analyzer to measure cellular electrical impedance (cell index value).
Developed a protocol for noninvasive and continuous monitoring of lymphocyte-target cell interactions.
Generated real-time killing curves to quantify lymphocyte-mediated cytotoxicity.

Main Results:

The impedance-based method enables real-time, noninvasive analysis of lymphocyte-mediated toxicity.
Continuous monitoring generates real-time killing curves, unlike traditional label-dependent assays.
The protocol facilitates accurate quantification of cytotoxicity.

Conclusions:

A label-free, impedance-based approach provides a powerful tool for real-time analysis of lymphocyte-mediated antitumor cytotoxicity.
This method overcomes limitations of traditional assays, enabling continuous and noninvasive monitoring.
The protocol is valuable for research and potentially clinical applications in cancer immunology.